Synthesis, characterization and application of new adsorbent composites based on sol-gel/chitosan for the removal of soluble substance in water.

In this work, new adsorbent composites from the silica precursor tetraethyl orthosilicate (TEOS) and chitosan have been successfully synthesized, denominated 20%Chi, 30%Chi and 40%Chi. The composites presented enhanced chemical and physical characteristics, with emphasis on the high surface areas between 374.94 m2/g to 886.31 m2/g. The application of the composites in the model system (TY - Tartrazine yellow dye), presented adsorption capacities dependent on the amount of chitosan in the composite (40%Chi > 30%Chi > 20%Chi). However, from the experimental data of the constituent materials, 30%Chi provided the greatest increase in the adsorption capacity in the monolayer, with values of 36%. This demonstrates that the amount of chitosan in the compound alters the arrangement of adsorption sites. The 30%Chi composite presented life cycle superior to 10 reuse cycles.

Synthesis of glutaraldehyde-modified silica/chitosan composites for the removal of water-soluble diclofenac sodium.

Composite materials are effective adsorbents for the removal of various types of contaminants, such as pharmaceutical products. However, they require improvement to achieve a good adsorption capacity. This study presents the development of a promising adsorbent: silica/chitosan modified with different proportions of glutaraldehyde, which involves the D-glucosamine units from chitosan. The developed materials were evaluated for their ability to remove diclofenac sodium. The adsorption data showed that the diclofenac adsorption efficiency increased with increasing degree of glutaraldehyde crosslinking. The equilibrium and kinetic data were well fit by the Liu and Elovich models, respectively, and the maximum adsorption capacity was 237.8 mg/g. Therefore, it can be assumed that the process is predominantly chemical and exothermic, with a high affinity between the adsorbents and diclofenac sodium. The adsorption mechanisms were investigated to better understand the interactions, and the predominance of covalent bonds with the self-polymerized glutaraldehyde was verified.

Bacterial biosurfactant increases ex situ biodiesel bioremediation in clayey soil.

The contamination of soils by oily compounds has several environmental impacts, which can be reversed through bioremediation, using biosurfactants as auxiliaries in the biodegradation process. In this study, we aimed to perform ex situ bioremediation of biodiesel-contaminated soil using biosurfactants produced by Bacillus methylotrophicus. A crude biosurfactant was produced in a whey-based culture medium supplemented with nutrients and was later added to biodiesel-contaminated clayey soil. The produced lipopeptide biosurfactant could reduce the surface tension of the fermentation broth to 30.2 mN/m. An increase in the microbial population was observed in the contaminated soil; this finding can be corroborated by the finding of increased CO2 release over days of bioremediation. Compared with natural attenuation, the addition of a lower concentration of the biosurfactant (0.5% w/w in relation to the mass of diesel oil) to the soil increased biodiesel removal by about 16% after 90 days. The added biosurfactant did not affect the retention of the contaminant in the soil, which is an important factor to be considered when applying in situ bioremediation technologies.

Synergistic effect of the activated carbon addition from leather wastes in chitosan/alginate-based composites.

In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW). The performance of these composites was verified through the adsorption of the textile dye Remazol Brilliant Blue R (RBBR). A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g. The synergy was due to the reduction in steric hindrance, with the adsorption capacity 1.2 times higher than expected. The material was regenerated with sodium hydroxide for 10 cycles. The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.

Effects of homemade biosurfactant from Bacillus methylotrophicus on bioremediation efficiency of a clay soil contaminated with diesel oil.

Despite constant progress in the understanding of the mechanisms related to the effects of biosurfactants in the bioremediation processes of oily residues, the possibility of antagonist effects on microbial growth and the production in situ of these compounds must be elucidated. The aims of this work were a) to evaluate the effects of the addition of a homemade biosurfactant of Bacillus methylotrophicus on the microbial count in soil in order to determine the possibility of inhibitory effects, and b) to accomplish biostimulation using media prepared with whey and bioaugmentation with B. methylotrophicus, analyzing the effects on the bioremediation of diesel oil and evidencing the in situ production of biosurfactants through effects on surface tension. The homemade bacterial biosurfactant did not present inhibitory effects acting as a biostimulant until 4000 mg biosurfactant/kg of soil. The biostimulation and bioaugmentation presented similar better results (p > 0.05) with the degradation of oil (~60%) than natural attenuation due to the low quantities of biostimulants added. For bioaugmentated and biostimulated soils, a decrease of surface tension between 30 and 60 days was observed, indicating the production of tensoactives in the soil, which was not observed in natural attenuation or a control treatment.

Biosurfactants during in situ bioremediation: factors that influence the production and challenges in evalution.

Research on the influence of biosurfactants on the efficiency of in situ bioremediation of contaminated soil is continuously growing. Despite the constant progress in understanding the mechanisms involved in the effects of biosurfactants, there are still many factors that are not sufficiently elucidated. There is a lack of research on autochthonous or exogenous microbial metabolism when biostimulation or bioaugmentation is carried out to produce biosurfactants at contaminated sites. In addition, studies on the application of techniques that measure the biosurfactants produced in situ are needed. This is important because, although the positive influence of biosurfactants is often reported, there are also studies where no effect or negative effects have been observed. This review aimed to examine some studies on factors that can improve the production of biosurfactants in soils during in situ bioremediation. Moreover, this work reviews the methodologies that can be used for measuring the production of these biocomposts. We reviewed studies on the potential of biosurfactants to improve the bioremediation of hydrocarbons, as well as the limitations of methods for the production of these biomolecules by microorganisms in soil.